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of in vitro isolated pancreatic islet cell populations by a novel cloning strategy.
Pancreatic islet cells can only be successfully isolated from rodent pancreata for a short period of time. The endocrine cell populations are depleted by immune-mediated destruction of the exocrine parenchyma and by the loss of the islets during the isolation procedure. We have therefore investigated the expansion of isolated islets in vitro. The factors involved in limiting the expansion of islet cell populations have been examined. Islets from newborn rats were plated as a single unit in tissue culture and the change in the number of cells was followed over an incubation period of 10 days. The be359ba680
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